BSc methods (2) thumbnail

Materials and methods

In my case of study, I used:

– a benthic fish, Parablennius sanguinolentus, as bioindicator

– nuclear abnormalities and micronucleous in eritrocytes as biomarker

in order to verify and quantify the damage caused by the presence of anthropogenical chemical pollutants (PAHs, benzo(a)pyrene, benzo(k)fluoranthene, heavy metals) in two sites of the Eastern Coast of Sicily (Italy).

The data were then combined with chemical analysis of water and sediments, bioaccumulation values of heavy metals in the muscles and skeletal abnormalities for an integrate environmental perspective.

For each sample, the methods included:

– data acquisition;

– blood sample with blood smear;

– muscles sample for heavy metals analysis;

– sample preservation for skeletal abnormalities analysis.

During the analysis on microscope the erythrochytes were divided in:

– normal (NE);

– abnormal (AE);

– micronucleous (MN).

Their relative frequence (FMN‰, FAN‰) was used in order to estimate the genotoxic damage.